Journal: Gland Surgery
Article Title: Tumor-derived mesenchymal progenitor cell-related genes in the regulation of breast cancer proliferation
doi: 10.21037/gs-23-387
Figure Lengend Snippet: Comparison of MPC and MSC morphology, surface markers, and their differentiation ability. (A) Morphological characteristics of MSCs and MPCs under microscopy (10×; scale bar: 200 µm). (B) Alizarin Red staining for osteogenic differentiation and Oil Red O staining or adipogenic differentiation (4×; scale bar: 500 µm; **P<0.01). (C) Flow cytometry showing the expression of positive surface markers of MPCs and MSCs (n=3): CD90, CD105, and CD73. (D) Flow cytometry showing the expression of negative surface markers for MPCs and MSCs (n=3): CD45, CD14, CD34, CD11b, and CD31. MSC, mesenchymal stem cell; MPC, mesenchymal progenitor cell.
Article Snippet: After MSCs and MPCs reached 100% confluence, the MSC completion medium was replaced with MSC adipogenic or osteogenic differentiation medium (ScienCell Research Laboratories) and cultured for 20 or 15 days.
Techniques: Comparison, Microscopy, Staining, Flow Cytometry, Expressing